Ultrastructural Changes Associated With Dexamethasone-Induced Ocular Hypertension in Mice

Ultrastructural changes associated with dexamethasone-induced ocular hypertension in mice.

PURPOSE To determine whether dexamethasone (DEX)-induced ocular hypertension (OHT) in mice mimics the hallmarks of steroid-induced glaucoma (SIG) in humans, including reduced conventional outflow facility (C), increased extracellular matrix (ECM), and myofibroblasts within the outflow pathway. METHODS Osmotic mini-pumps were implanted subcutaneously into C57BL/6J mice for systemic delivery of...

Ocular changes in rabbits with corticosteroid-induced ocular hypertension.

Rabbit eyes with steroid-induced ocular hypertension were investigated in order to evaluate the histochemical abnormalities in the chamber angle region. The right eye of 14 rabbits was treated by dexamethasone 1% 3 times daily for 3 to 5 weeks. The eyes were stained by haematoxylin-eosin, periodic acid Schiff, fibrin, colloidal iron, and alcian blue with and without hyaluronidase. All treated e...

Dexamethasone-induced ocular hypertension in perfusion-cultured human eyes.

PURPOSE Glucocorticoid administration can lead to the development of ocular hypertension and corticosteroid glaucoma in a subset of the population through a decrease in the aqueous humor outflow facility. The purpose of this study was to determine whether glucocorticoid treatment can directly affect the outflow facility of isolated, perfusion-cultured human eyes. METHODS The anterior segments...

Ultrastructural and biochemical changes associated with pyrrolizidine-induced hepatic megalocytosis.

Laser-induced ocular hypertension in albino CD-1 mice.

PURPOSE To establish a laser-induced model of ocular hypertension (LIOH) in albino CD-1 mice and to characterize the sequence of pathologic events triggered by intraocular pressure (IOP) elevation. METHODS LIOH was induced unilaterally in CD-1 mice by laser photocoagulation of limbal and episcleral veins 270 degrees to 300 degrees circumferentially, sparing the nasal aspect and the long cilia...